This pipeline demonstrates how to accurately identify these cells and how to measurements cellular parameters such as morphology, count, intensity and texture. Human cells: Human HT29 cells are fairly smooth and elliptical. (g) Speckles: fluorescent foci of phospho-Histone2AX induced by 2 Gy of irradiation in human U2OS cells disappear at timepoints as the cells recover. These pipelines are made for simple cellular and tissue image assays, and include some basic measurements. Totals do not equal 100% due to slight overlap between compartments. (f) Protein localization: the mean intensity of NFκB staining in the cytoplasm and the nucleus is shown in response to TNFα in human MCF7 cells (top). Wild-type human HT29 nuclei that stain positively for phospho-histone H3 tend to be smaller than phospho-H3-negative cells (right). (e) Phospho-protein content: human HT29 cells treated with RNAi reagent against Polo kinase have an increased percentage of nuclei with high phospho-H3 staining compared to wild-type cells, consistent with a mitosis-stalled phenotype (left). elegans, only the boxed region of interest was analyzed. The histone content is decreased by roughly half in each daughter nucleus after division. elegans) content is shown near each nucleus in arbitrary intensity units. cerevisiae) or GFP-histone H2B (HeLa and C. (d) Chromatin content in time lapse movies: GFP-histone H4 ( S. RNAi-targeted samples were also as expected for Aurora kinase B (polyploid), Mad2 (fairly normal cell cycle distribution), String (4N-enriched), and Anillin and Cyclin A (both polyploid). The cell cycle distributions are as expected, with the 2N peak being predominant in the wild-type human sample, whereas most wild-type Drosophila nuclei are known to have 4N DNA content. (c) DNA content in cell populations: measurements are shown for human HT29 cell populations (1 image for each RNAi condition, left) and for Drosophila Kc167 cell populations (1,750 images for each RNAi condition were combined, right). (b) Cell size: CellProfiler's cell area measurements are comparable to those of a Coulter particle counter for Drosophila Kc167 cells, for wild-type (no dsRNA) and RNA-interference induced samples. Example images and CellProfiler outlines for these cell types are shown in Figure 1e. (f) Protein localization: the mean intensity of NFB staining in the cytoplasm and the nucleus is shown in response to TNF in human MCF7 cells (top). For images of Drosophila Kc167 cells with various genes knocked down by RNAi (right), the two researchers' counts varied by 16% and CellProfiler's counts were within 17% of their average. (a) Cell count: for a set of 6 images of wild-type human HT29 cells (left), two researchers' counts varied by 11%, and CellProfiler's counts were within 6% of their average. See also MeasureObjectIntensity, MaskImage.Validation of CellProfiler for many cellular phenotypes. TotalArea: Number of pixels measured, e.g., the area of the image.UpperQuartileIntensity: The intensity value of the pixel for which 75% of the pixels in the object have lower values.LowerQuartileIntensity: The intensity value of the pixel for which 25% of the pixels in the object have lower values.MinIntensity, MaxIntensity: Minimum and maximum of pixel intensity values.The MAD is defined as the median(|x i - median(x)|). StdIntensity, MADIntensity: Standard deviation and median absolute deviation (MAD) of pixel intensity values.MeanIntensity, MedianIntensity: Mean and median of pixel intensity values. TotalIntensity: Sum of all pixel intensity values.This "maxmimum possible" value is defined by the "Set intensity range from" setting in NamesAndTypes see the help for that setting for more details. Keep in mind that the default behavior in CellProfiler is to rescale the image intensity from 0 to 1 by dividing all pixels in the image by the maximum possible intensity value. Also, it is important to note whether you are reporting either the mean or the integrated intensity, so specify "Mean intensity units" or "Integrated intensity units" accordingly. Note that for publication purposes, the units of intensity from microscopy images are usually described as "Intensity units" or "Arbitrary intensity units" since microscopes are not calibrated to an absolute scale. If the image has a mask, only unmasked pixels will be measured. The user can measure all pixels in the image or can restrict the measurement to pixels within objects. This module will sum all pixel values to measure the total image intensity. Measure Image Intensity measures the total intensity in an image by summing all of the pixel intensities (excluding masked pixels).
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Bun B and Ying Yang Twins urge the ladies to get on the dance floor and show off their moves. Ying Yang Twins are a classic representation of the crunk style of music that was popular in the early 2000s. So get it get it get it get it get it get it girl I came to the club to put some money in your world Hold that shit girl show me what you got thereīitch get crunk let me see you hit a splitīop your ass up and down like you ridin on a dick You Still in the booty club naked shake it So your ass get crunk show me all of your skills Start poppin that pussy and then gettin it low So its best you get your ass on the floor GET CRUNK for a nigga get loose with it bitch Lick the lolly pop while i play with the puddin (YEAH)ĭon't be scared, its all good, go, get it Have you doin things that your last man couldn't You need to let me take that thing with me back to the padĬoz when i put it on you betchya won't be madĪnd its all night long we don't need no sleep Nigga leave the club with his print showin in his pantsĭamn! look at that face look at those eyes I can't make her mine but i could drop a dime She playin with my mind see the bitch is fine These girls in the club you better come on with itĭAMN I like the way she put that thing on a nigga What the hell you waitin on your ass better get it These hoes is for the takin no clothes that mean they naked Pussy poppin and shakin bringin home the bacon Sight for sore eyes these hoes be deliverin Magic City, Monday, thats the crunk night Native is the Windows default of a single cursor. There are three different modes in MouseMux Native Mode, Switched Input, and Multiplex Input. However, we are unsure if the project is still ongoing as there has been no activity since May 2019. Currently, MouseMux is still in beta and betas are free to use. MouseMux is by a former lead engineer at TeamPlayer. TeamPlayer 2 works reasonably well but we did encounter a few minor issues, which might be expected of a program this old. The other cursors can be moved freely and you simply left click to take control and make that mouse active. The white cursor with a colored square is active and can be used for left or right click actions. On screen there will be up to three mouse cursors, each with its own color. During launch, 64-bit users will be shown a 64-bit beta warning which can be ignored. Make sure you have plugged in the mice and keyboards before launching this TeamPlayer as it doesn’t recognize new devices while running. The one available checkbox in the user interface is to check for updates which doesn’t work. That’s a useful advantage over TeamPLayer4 Lite if you need the third user. It’s free for personal use, doesn’t expire and accepts up to three mouse/keyboard users at once. This older beta version of TeamPlayer dates way back to 2008 but we tried it in the latest Windows 10 64-bit and it still works. Here we show you seven free programs that allow more than one mouse cursor on the screen at once. While this is by design, you might want to have more than one user interact with the desktop at the same time or have one user on one monitor and another have a separate cursor on a second monitor.įunctions like these are not possible in Windows without using third party software. All the connected devices will then be able to move the single cursor. There are several ways to move the cursor around, including laptop touchpads, trackballs, mice, and even keyboards.Įven if you plug in more than one device that can move the cursor around, you will only ever get one visible cursor on the screen. This tells you where the active area is to perform specific actions at any given point in time.
The entire tiny house is yours! For food: You have access to all kitchenware, myriad spices, anything in the pantry, cupboards or fridge, coffee/coffee maker, etc. More about the space: This tiny house is on a vast 80-acre property! My friends John and Audrey live on the property about 400 feet north in a farmhouse on the other side of the grove of trees, and I live on the property in the neighboring teeny tiny house! We are building a tiny house community! We all love Jesus, and have a passion for seeing Him heal and restore people. I know this type of thing isn’t for everyone, though most people seem to adapt just fine. But please be conscious of this fact before you book, and know to expect it. Please be aware that like most tiny housers, I have a composting toilet-it’s environmentally friendly, saves a ton of water, and I love it! (I also built it!). My desire for everyone who stays in my home is first, that you feel valued and valuable, and second, that you feel and enjoy the peace that I feel in this space. Since I was custom building it for myself, I had to include a few luxuries and wish-items! My favorite features are: the heated floor throughout the bathroom and bedroom, the fire place, the big back deck/carport, in-ceiling speakers throughout the house, the electric fireplace by the master bed, the hardwood oak living room/kitchen floor, the reflective ceiling tiles (create an illusion of space and 'bigness'), the look of the storage stairs, the off-grid water system, the barn wood that I incorporated throughout the house, and the huge picture window in the living room. Once inside, it feels bigger than you'd think, but it's still technically only 342 sq feet on the bottom level. It turned out exactly how I wanted! This house is one of those "Not-so-tiny" tinies. I wanted to look at nature all the time, and tried to "bring the outside in". I designed and built this house myself, and wanted two things in order to give it an open, bright ambiance: High ceilings and big windows. My tiny house has a wide-open feel first because of the landscape (rolling hills and NE plains), and next because of all the windows that let that nature in. All on a spacious property of rolling hills and spectaular views. The entire footprint of this home is 12x32 (not including decks)-but for a tiny house, it actually is quite spacious! Huge windows with beautiful views of the Nebraska countryside, fully equipped kitchen with stainless steel appliances, in-ceiling speakers, wifi, Netflix/Amazon Prime, large back deck, front deck, 3'x5' shower, washer/dryer, indoor fireplace (turned off during summer months), outdoor propane fire pit, oak floors, reclaimed barnwood accents throughout. "Tiny House Stephanie Bock" (a 2 minute video, the first one that comes up). To watch a short video showing the space, search this phrase on Youtube: Come in after enjoying nature and relax with a glass of wine in an elegant, modern tiny house. Enjoy a breath-taking view of the stars from the outdoor hot tub. Lounge in a hammock, meditate or write, explore the 80-acre property, or relax on the back deck by the outdoor fire pit. Sip a cup of coffee while gazing at greenery from the living room or back deck. Martinsburg, NE Discover the simple beauty and tranquility of Living Tiny while surrounded by soft, green hills.We have a large area for children to run and play including three different places in the sandbox. We are 15 miles from Sioux Falls And 6 miles from Garretson (where you have the opportunity to visit Devils Gulch, Split Rock Park, or Palisades State Park!) We are located 11 miles north off of interstate 90. If your family is traveling with four wheelers or dirt bikes we have a large area to explore along with a man-made track that is on the edge of the yard. Next to the picnic shelter is a large area to have a bonfire where you can grill food or just relax around and enjoy the scenery!Īlong with your stay, we provide being big boards and bags for your entertainment along with a stereo for music. The picnic shelter is equipped with a loft along with refrigeration, stove, oven, microwave, soon, shower, and a toilet. We reserve the right to prohibit fires if we feel it’s too dry or windy.Ī picnic shelter is available to use for assistance if extra bedding or space is needed. There are many RV spots with the power along with a pool!įirewood is available on site. You can wander around the pasture and creek during your stay. The goats are super friendly and love to be petted or fed. We have pet goats, cows, chickens, dogs, cats, and kittens! Depending on when you come you could witness baby chicks hatching or baby goats being born or bottled. Our 13.5 acres has a lovely view of the pasture, creek, and surrounding farms. Garretson, SD Welcome to the Larson Homestead!. Have also had memory issues that may or may not have been resolved by a battery replacement - always a potential issue with this vintage.īought and returned the Ethernet iConnectivity unit after discovering that it needed an attached host PC to be running to keep some watchdog program on the network for the network MIDI to work, (which imho defeats the point of using a peer-to-peer protocol like ETH), serious non-starter, returned to sender.Īm anxiously awaiting another ethernet RTP-MIDI offering from Alyseum, am hopeful that MIDI over ethernet will prove to be the "one-true" future protocol to allow a transport environment that is friendly to a mixture of MIDI, OSC and the newer high bandwidth uses of MIDI that are begining to be explored, ie. These days am using the MOTU Expresss XT and, while it works ok for the most part, I have found the drivers/software-interface to be very hit-or-miss on my Win10 system - (it worked normally at first but lately claims the device isn't detected, but somehow the changes still end up making it to the device after the program spins out for a while). 48 input line mixer (31 pages) Mixer Roland AeroCaster VRC-01 Owners Manual. (programming the channels in hexadecimal is a bonus!) Have had several older JL Cooper units and they worked reliably for years for a crazy octopus nest of cables connecting up a bunch of 90's boxes, they are decent options if you don't also need a PC interface. Gravyface wrote:Anyone have any experience with the MOTU Express XT? I don't remember how much it was, but under $100. I also happened to see a 360 Systems MIDI Patcher at Lewiston Music in Lewiston, NY a few weeks ago and I bet they still have it. Insanely useful - I use mine pretty much every day. This unit is designed for those who have many MIDI controlled electronic instruments, and want to be able to switch among which one is the master controller. No parameters or buttons or menus, just eight sliders. Does one thing, and really well - simple, solid build, intuitive MIDI matrix. I assume you could daisy-chain more patchbays quite easily. I have Kawai and Roland interconnected with one pair of I/O so it's possible to route any port anywhere. Roland A880 - 8x IN, 8x OUT, more advanced features like routing preset storage, MIDI merge on two selected channels (one can carry MIDI clock), immediate control for basic routingĪlyseum AL-88c - 8x IN, 8x OUT, very small with tiny footprint - good for portable setups, requires software for configuration but runs standalone with flashed routing config, can act as MIDI interface, really flexible Cube48 wrote: Kawai MAV-8 - cheap, 4x IN, 8x OUT, simple but immediate The market is full of arts and crafts, including paintings, pottery, and jewelry there are even health and body products and prepared food. Starting off your Fort Smith adventure with a visit to the free, open-air Rivervalley Artisan Market is a great way to meet a few locals, see some fantastic locally made products, and have an all-around good time. Rivervalley Artisan Market Source: Rivervalley Artisan Market / Facebook Rivervalley Artisan Market The city is an especially exciting place for amateur historians interested in learning more about the Civil War.īelow are 15 fun and entertaining things to do in Fort Smith. Known for its natural beauty, the state of Arkansas was once a French territory called New France, and part of what would later become the state of Louisiana. Straddling the banks of the Arkansas River, the city has a history dating back to the frontier era when a number of Native American groups inhabited the area. The Arkansas River flows through the park as well as several creeks and a lake, which offer prime fishing and other watersports opportunities.With nearly 90,000 residents, Fort Smith is Arkansas’ second largest city behind Little Rock. The park is in a beautiful setting and the campsites have electric hookups with access to picnic areas, shower facilities, playground, and boat ramp. You can also consider bringing the Fort Smith rental RV to Springhill Park Campground. Guests have access to cable TV and WiFi, and the park is close to great shopping and restaurants. There’s a boat ramp with dock and a playground close to the campsites. The park is open year round and is centered around family fun. They have large sites that can accommodate oversized trailers and motorhomes. They have full hookups and are close to several state parks and historic sites of interest.Īnother popular place for relaxation and camping is Parkridge Campground. The Overland RV Park is another park in the area, and it caters to all guests, both long and short term. The park is close to many restaurants, historical sites, and local shopping. Fort Chaffee RV Park is a full-service park that offers spacious campsites with full hookups for military, reserves, retirees and DOD employees. There are tons of options when it comes to finding a place to park the Fort Smith RV rental. The park features campsites with full hookups as well as a seasonal swimming pool, tennis court, ballfields, gift shop and more. There are three hiking trails to explore with a chance to spot the rare Ozark trillium flower. The gentle waters are perfect for floating, fishing and relaxing. It’s one of the most popular parks in the state. Another great place to take the Fort Smith rental camper is Withrow Springs State Park. There is an archery range on site, plus it is the only state park that allows regulated hunting during season. The park’s trail system stretches across 36 miles for hikers, bikers, and equestrians. It’s the biggest state park in the state, covering more than 12,000 acres of pristine Ozark countryside on the southern shore of Beaver Lake. Head north and you’ll find Hobbs State Park-Conservation Area. Bring the Fort Smith motorhome rental and do some camping under a gorgeous starry sky. There are miles of trails on land as well. Float down the river and you’ll find rushing rapids and serene clear pools surrounded by lush forest and towering bluffs. It’s one of the last undammed rivers in the state and stretches freely for more than 150 miles. The Buffalo National River brings in visitors from all over the world. There are also great places for swimming, boating, fishing and hunting during game season. The area is also a popular place for hiking, mountain biking and horseback riding, with hundreds of miles of trails to explore. The forest covers more than a million acres and features the tallest mountain in Arkansas, Mount Magazine, and the Blanchard Springs Caverns, a breathtaking and alive underground cave system. For some of the most beautiful wilderness and best camping in the state, head over to the Ozark National Forest with the Fort Smith camper rental. Arkansas is The Natural State, and with an RV rental near Fort Smith you’ll be able to experience all that natural wonder with ease. Failure to follow this rule exposes employees to a potentially dangerous condition should the known malfunction recur. The work flow stops at any point if the issue can be resolved, but in order to promote employee safety, it is essential to adequately troubleshoot and repair any malfunctioning equipment before returning it to service.
But Macube can make such time-wasting work to be quicker for developing the specific Duplicate Finder. It takes both your time and effort, and you have to focus on finding and sorting out the items one-by-one. ConclusionĬleaning duplicate photos manually is not an easy task. Next time when you have other duplicate photos to delete, you can return to proceed with the cleanup directly. One-click on the Delete All button in the upper right corner for clearing them up.Īfter such duplicate photos cleaning process, the Smart Album will be saved in the Photos app's sidebar. After deleting the photos, please turn to Recently Delete in the left sidebar. Right-click on it and directly tap the Delete button. Please select the photos you wish to delete. For example, you can sort out all the photos that is marked as favorite, and you can add more filters like names to narrow down the scope and identify duplicate photos. Set a name for the album and don't forget to set its filter criteria as well. In the Photos app, you need to go to File > New Smart Album. You just need to create a smart album for assisting you. On Mac, people can utilize a smart feature for deleting duplicate photos in the Photos app manually. Photos will be the place where most duplicate photos are saved. Manually Clean Up Duplicate Photos in Photos App Finally, empty your trash and all the duplicate will be removed permanently. Control-click for moving the duplicate photos to trash directly. In the Kind drop-down menu, you will find all the duplicate photos across different folders listed here, so you can directly choose those you don't need. In the newly-created folder, tap This Mac and click on the + icon in the upper right corner. Open Finder and go to File > New Smart Folder. Thanks to Mac's Smart Folder function, it helps to sort such files by specific criteria, making it easier to find duplicate photos for deleting. You may have collected many duplicate photos overtime on Mac, and they are not saved in the same folder. (Or you can take them all!) Use Finder to Find and Remove Duplicate Photos on Mac Now choose the option you prefer to manipulate. This part will introduce 2 more ways for you to find and delete them manually. Macube will bring a cleaner Mac to you when it ends the duplicate photos cleaning job! 2 Ways to Find and Delete Duplicate Photos Manuallyįor double checking whether there may have more duplicate photos required to be cleaned up on Mac, some people may wish to manually check up Mac for finding and deleting duplicate photos. Subsequently, only tap the Clean button to proceed cleaning up them.Īfter clicking on the Clean button, you need to do nothing but just wait for the cleaning process to complete. When Macube ends its work and all the duplicate items are listed now, please select the photos or images you wish to clear up for freeing Mac storage. Then Macube will automatically proceed to locate and list the duplicate items stored on the Mac computer. Turn to Duplicate Finder on the left panel and utilize only one click to scan your Mac. Macube has been developed a professional duplicate files scanning tool to assist resolve such issues, so it will facilitate duplicate photo deleting to be more convenient. You don't have to follow up the sorting process but simply select from the scanned results for deciding the duplicate photos to delete. Macube is able to smartly scan your Mac for sorting out the duplicate images, including those imported ones or originally taken photos with only one shot. So, a more efficient way to find and remove duplicate photos automatically is to make full use of some third-party Mac cleaning apps, and Macube can be one of your options. You still can do nothing to the duplicate photos that have already been stored on your Mac. Therefore, it is able for you to find and remove these auto-sorted duplicate photos conveniently on Mac directly.īut the feature is limited because it's only available when you are importing photos from external. How to Find and Remove Duplicate Photos AutomaticallyĬonveniently, the Photos app of Mac will automatically detect duplicate photos as you import them from the external place to Mac's camera roll. 2 Ways to Find and Delete Duplicate Photos Manually.How to Find and Remove Duplicate Photos Automatically. Now, connect the 3/16″ lines that you drew both vertically and horizontally. Your letter should look something like this: Identify the remaining corners of your letter, and draw more 3/16″ lines that are parallel to your diagonal lines. “rolling ruler”) because you can line up the ruler with a diagonal line and easily roll it forward or backward. I find it easiest to draw 3D letters with a parallel glider (a.k.a. Then, draw along the edge of the ruler to make a pencil line that extends 3/16″ (~ 5mm) from the corner of the letter. Line up your ruler with that corner such that the ruler is parallel to the slant lines you drew before. To start drawing your shadows, begin with the lower left corner of your first letter. As such, shadows will appear to the lower right of the letters. To make these letters 3D, I’m assuming that there’s a light source coming from the upper left corner of the page. To get started with your shadow, use a pencil to lightly draw several diagonal, parallel lines over your phrase. Our goal here is to make these letters look real, like they’re sitting on the page. I chose “EAT MORE KALE” because that’s exactly what I need to do! My garden is overrun with it. For efficiency’s sake, it’s always best to commit to a couple of words versus a full sentence. Remember that it takes some time to draw 3D letters. This is a thicker version of Sans Serif lettering from the Premium Hand Lettering Worksheet Set. If you’d like to write at a more leisurely pace, try writing your own letters (preferably aided by pencil guidelines), as I’ve done here. If you’re pressed for time, you can type out your phrase in a word processing program, print, and trace the letters. So, start by drawing out a short phrase using thick letters. Letters will only look 3D if you give them a shadow, and in order to cast a shadow, the letters need to be thick. In this article, I’ll teach you how to draw 3D letters using a ruler, a pencil, and a pen. In the course of the tutorial, I ask you to write 3D letters … and yet, no 3D letter tutorial existed on the TPK website! That changes today. A couple of weeks ago, I wrote a tutorial called How to Hand Letter Like a Pro. You can reach out to the website’s operators by using the following email Screen-reader and keyboard navigation If you’ve found a malfunction or have ideas for improvement, we’ll be happy to hear from you. This application remediates the website’s HTML,Īdapts Its functionality and behavior for screen-readers used by the blind users, and for keyboard functions used by individuals with motor impairments. We utilize an accessibility interface that allows persons with specificĭisabilities to adjust the website’s UI (user interface) and design it to their personal needs.Īdditionally, the website utilizes an AI-based application that runs in the background and optimizes its accessibility level constantly. This website utilizes various technologies that are meant to make it as accessible as possible at all times. To all people: blind people, people with motor impairments, visual impairment, cognitive disabilities, and more. Complying with those guidelines helps us ensure that the website is accessible These guidelines explain how to make web content accessible to people with a wide array of disabilities. To fulfill this, we aim to adhere as strictly as possible to the World Wide Web Consortium’s (W3C) Web Content Accessibility Guidelines 2.1 (WCAG 2.1) at the AA level. We firmly believe that the internet should be available and accessible to anyone, and are committed to providing a website that is accessible to the widest possible audience, |